Research Article

Anti-anxiety effects in mice following acute administration of Ficus Thonningii (wild fig)

Aduema W*, Akunneh-Wariso C, Ejiofo DC and Amah AK

Published: 11 September, 2018 | Volume 2 - Issue 1 | Pages: 040-047

The effect of acute administration of ethanol extract of F. thoningii on anxiety and fear in Swiss white mice was studied. 30 adult Swiss white mice of both sexes were randomly divided in to three groups of 10 mice each. Group1 served as the control and was administered normal saline only. Group 2 (low dose group) was administered 10mg/kg ethanol extract of the F. thoningii, while group 3 (high dose group) was given 20mg/kg of the same extract. All animals were allowed food and water ad libitum. Neurobehavioral parameter was assessed using the light/dark transition box. The analysis of variance (ANOVA) was used to test for variability within and among groups. Results were expressed as Mean ±SEM (standard error of the mean) and probability level p<0.05 was accepted as significant. The result showed that the frequency of transition in the light/dark transition box was significantly increased in the test groups (p<0.05; p<0.01).Similarly, the Light Box Duration was also significantly increased (p<0.01) in the low and high dose groups respectively. However, the Dark box duration was significantly decreased (p<0.05; p<0.01) in the low and high dose groups compared to control. This index showed a decreased level of anxiety and fear in the test groups. This was followed by a corresponding trend of decreased frequency of stretch attend posture and duration of freezing in the light/dark transition box (p<0.01; p<0.001) compared to the control. Summarily, acute administration of ethanol extract of F. thonningii causes calmness and sedation in moderate and high doses. It is therefore likely that it reduces aggression. If the result from this finding is extrapolated to humans, F. thoningii could be used to reduce anxiety disorders.

Read Full Article HTML DOI: 10.29328/journal.ida.1001009 Cite this Article Read Full Article PDF


Ficus thoningii; Swiss white mice; Light/dark transition box; anxiety


  1. Ajayi AO. Antimicrobial nature and use of some medicinal plants in Nigeria. African Journal of Biotechnology 2008; 7: 595-599. Ref.: https://goo.gl/yDL3F6
  2. Usman H, Abdulrahman FI, Usman A. Qualitative phytochemical screening and in vitro antimicrobial effects of methanol stem bark extract of Ficusthonningii (Moraceae) AJTCAM. 2009; 6: 289–295. Ref.: https://goo.gl/svYm8s
  3. Orwa C, Mutua A, Kindt R, Anthony S. Agroforestree database: a tree reference and selection guide. 2009; Ref.: https://goo.gl/vzYkXY
  4. Agroforestry Tree Database. 2011; : https://goo.gl/2j4GRd
  5. Hines DA, Eckman K. Indigenous multipurpose trees of Tanzania Uses and economic benefits for people. FAO Corporate document repository. 1993; Ref.: https://goo.gl/33kXX9
  6. Hyde MA, Wursten B. 2011 Flora of Zimbabwe. Ref.: https://goo.gl/vx4fLf
  7. Schmidt E, Lutter M, McCleland W. Trees and shrubs of Mpumalanga and Kruger National Park. Jacana, South Africa. 2002; 80 Ref.: https://goo.gl/SdzCy6
  8. Danthu P, Solovier P, Gaye A, Sarr A, Seck M, et al. Vegetative propagation of some West African Ficus species by cuttings. Agroforestry Systems 2002; 55: 57-63. Ref.: https://goo.gl/4b5KrW
  9. Beglinger C. Overview: cholecystokinin and eating. Current Opinion in Investigational Drugs. 2002; 3: 587-588. Ref.: https://goo.gl/knjoau
  10. Burrows J, Burrows S. Figureures of Southern and South-Central Africa, Umdaus Press. Hatfield, South Africa. 2003; 62-100. Ref.: https://goo.gl/vUQGcs
  11. Tegbe TSB, Adeyinka IA, Baye KD, Alawa JP. Evaluation of feeding graded levels of dried and milled Ficusthonningii leaves on growth performance, carcass characteristics and organ of weaner rabbits. Pakistan J Nutr 2006; 5: 548–550. Ref.: https://goo.gl/Ds8ErU
  12. Jansen O, Angenot L, Tits M, Nicolas JP, De Mol P, et al. Evaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties. J Ethnophamacol. 2010; 13: 143–150. Ref.: https://goo.gl/VRiXrJ
  13. Titanji VPK, Zofou D, Ngemenya MN. The antimalarial potential of medicinal plants used for the treatment of malaria in Cameroonian folk medicine. AJTCAM. 2008; 5: 302–321. Ref.: https://goo.gl/BbX9Nm
  14. Bamikole MA, Ikhatua UJ. Nutritional evaluation of Ficus thonningii-Panicum maximum mixtures in West African dwarf goats. Nutrition and Food Science. 2010; 40: 280–288. Ref.: https://goo.gl/uCkQ83
  15. Igoli JO, Tor-Anyin TA, Usman SS, Oluma HOA, Igoli NP. Folk medicines of the Beneu valley of Nigeria. In: Singh VK, Govil JN, Hashim S, Sing G, 2002. Recent progress in Medicinal plants, Ethnomedicine and Pharmacognosy. Raleigh, NC: Science Technology Publishers. 2010; 327–338. Ref.: https://goo.gl/BGLmpi
  16. Mathieu G, Meissa D. Traditional leafy vegetables in Senegal. Diversity and medicinal uses. AJTCAM. 2007; 4: 469–475. Ref.: https://goo.gl/ZwTDmi
  17. Acamovic T, Brooker JD. Biochemistry of plant secondary metabolites and their effects in animals. Proceedings of the Nutrition Society. 2005; 64: 403-412. Ref.: https://goo.gl/hV7wrf
  18. Cowan MM. Plant products as antimicrobial agents. Clinical Microbiology Review 1999: 12: 564-582. Ref.: https://goo.gl/qe6xBg
  19. Ahur VM, Madubunyi I, Adenkola AY, Udem SC. The effect of acetyl acetate extract of Ficus thonningii (Blume) leaves on erythrocyte osmotic fragility and hematological parameters in acetaminophen-treated rats. Com Clin Pathol. 2010; 10: 1107–1111. Ref.:
  20. Cryan JF, Mombereau C. In search of a depressed mouse: utility of models for studying depression-related behaviour in genetically modified mice. Molecular Psychiatry. 2004; 9: 326-357. Ref.: https://goo.gl/4VUynB
  21. Nilson R. A qualitative and quantitative risk assessment of snuff dipping. Regulatory Toxicology and Pharmacology. 1998; 28: 1-16. Ref.: https://goo.gl/dmDPrA
  22. Osim EE. Neurophysiology. Calabar. University of Calabar Press. 2008; 24-27.
  23. Aduema W, Munach I, Akunneh-Wariso C. Effect of Ethanol Extract of Nymphaea lotus (Water Lily) on fear and anxiety Behavior in mice. Annals of Clinical and Laboratory Research. 2018; 6: 223. Ref.: https://goo.gl/jVfhML
  24. Adolphs R, Gasselin F, Buchanan TW, Tranel D, Scgyns P, et al. A Mechanism for impaired fear recognition after amygdala damage. Nature. 2005; 433: 68 -72. Ref.: https://goo.gl/DNRAek


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